What is CRISPR/Cas9?
CRISPR/Cas9, originally discovered in 1987 by a team of Japanese scientists and later refined by Jennifer Doudna in 2012, is a gene-editing tool that can cut and paste any genomic sequence, either in vitro or in vivo. It’s a system that relies on clustered regularly interspaced short palindromic repeats (CRISPR) to recognize foreign DNA and is mainly used in bacteria to fight off viral infection. This tool has garnered a lot of attention recently as researchers have tailored CRISPR/Cas9 to edit animal genomes in ways that were previously impossible or inefficient, revolutionizing genetic and biomedical research. CRISPR/Cas9 has become a crucial resource for labs who require stable cell lines or mice with knockouts, knock-ins, or gene mutations, able to drive constitutive gene activation or to edit micro-RNA and long-noncoding RNA.